Phew… 

Our labs worked quite hard in the last few days in order to manage the high run on LIGHTrun.

Since only a few days GATC Biotech provides the new LIGHTrun, the most convenient single read sequencing service. LIGHTrun makes everything much easier, less complicated and so quick – our customers are obviously pleased!

NEW about it: You send us the premix of DNA and primer.

Just order your barcodes, mix DNA and primer, attach the barcodes to the premix tubes and go! 

Get three sequencing barcodes for free www.gatc-biotech.com/lightrun!

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Puh…

Unsere Labore mussten in den vergangenen Tagen ganz schön ackern, um den großen Ansturm auf den neuen LIGHTrun zu bewältigen.

Seit wenigen Tagen bietet GATC Biotech mit dem LIGHTrun einen neuen und total bequemen Single Read Sequenzierservice an. Jetzt geht alles noch viel schneller und unkomplizierter – und unsere Kunden sind begeistert!

Das NEUE daran: Sie schicken uns den Premix von DNA und Primer.

Einfach Barcodes bestellen, DNA und Primer mischen, Premix-Tube mit Barcode bekleben und an GATC senden.

LIGHTrun gratis testen unter www.gatc-biotech.com/lightrun!

We adjusted our sample requirements, added primer conditions and recommendations for sample delivery!

Check it out:

http://www.gatc-biotech.com/en/info-center/dna-konzentrationund-volumen.html

“Never change a running horse” is an old English saying. Well, we believe more in Georg Christoph Lichtenberg’s maxim, who once said more than 200 years ago:  “I don’t know whether it is getting any better if it becomes different. But it has to become different if it should be any better.” Since anything can always be improved as there is no such thing as perfection, we decided to create for you a brand new myGATC order system. And, on top, we will change our service portfolio starting 21 January, 2011.

Don’t be frightened. Actually, we made things easier. In the past, you had to know beforehand what article you planned to order. Now, you can order without knowing anything about our services. We will lead you through the order process in very logical steps. First you decide how to send your samples (in tubes or plates), then what you will provide (plasmids, PCR fragments, BAC, PAC, cosmids or ready-to-load reactions), and whether your delivered samples need purification or not. We have created a common spreadsheet, similar to an Excel table, in which you will define your sequencing reactions. You can even drag and drop barcodes visually into your order spreadsheet.

However, we agree that any change requires some learning in the beginning. To keep that to a minimum, we prepared a trailer to guide you through the new order process and set up a sneak preview so that you can explore the new system quickly and at your convenience.

The service portfolio required some modernization, too. There are some new services and some which did not make it to 2011. You can now send us BAC, PAC, cosmids or ready-to-load reactions in full or half 96 well plates. However, service articles BigRun (in tubes), SequenceYourself (in tubes), the GenomeRun and the complete range of primer walking services had to go. RIP. Nevertheless, if you are just planning a primer walking project – before you get annoyed please get in touch with us. We’ll find a solution.

We managed to decrease the required volume of DNA and custom primers from 30µl to 20µl. This will still be sufficient for up to eight reactions. However, we had to decrease the storage time of DNA and primers you provide from four weeks to five working days once the order is completed.

Here are the changes in an overview:

We continue to update myGATC 2011 during the year. Help us to develop innovative solutions that will make your lab life a bit easier. Please discuss with us your ideas, suggestions and criticism right here on the GATCBlog.

Best regards,
Jochen Schäfer

Orange-farbene Barcodes sind erst auswählbar im Pull-down-Menü nach einer Barcode Aktivierung.

Bitte die non-prepaid und Primer Barcodes vor Benutzung aktivieren unter: MyGATC account, Barcode Aktivierung (linke Seite).

The concentration of the template depends on the type of template.

For PCR-products a concentration between 10 and 50 ng/µl will be needed.

Plasmids should have a concentration between 30 and 100 ng/µl.

Der Unterschied zwischen den orangen und grünen Barcodes besteht in der Art der Bezahlung des Sequenzierauftrages.

Diese Barcodes gelten für die Sequenzierung einzelner Proben in Eppendorf-Tubes. Barcodes erleichtern die Arbeit im Labor, da Proben eindeutig einem Auftrag zugeordnet werden können.

Mit den grünen Barcodes (prepaid barcodes) wird die Leistung im Voraus bezahlt und mit den orange-farbenen Barcodes im Nachhinein. Vorteile der grünen Barcodes: geringerer Preis pro Sequenzierung, geringer Verwaltungsaufwand, Übertragung an Kollegen möglich, keine Barcode-Aktivierung notwendig. Bei orange-farbenen Barcodes wird nach jedem abgeschlossenen Auftrag eine Rechnung an Sie verschickt.

Pour reussir son sequencage, il est important de choisir les bon primers.

Dans le cas ou ces derniers doivent etre dessines, certains criteres sont a respecter tel que la temperatur d annealing, la longueur des primers ainsi que leur homogeneite en terme de bases qui les composent.

Die Konzentration hängt von der Art des Templates ab.

Für Plasmide gilt eine Konzentration von 30 - 100 ng/µl. Bei PCR-Produkten sollte die Konzentration zwischen 10 und 50 ng/µl liegen.

Für Primer gilt generell eine Konzentration von 10 pmol/µl.